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https://www.ias.ac.in/article/fulltext/jgen/095/02/0273-0281

random amplified polymorphic DNAs; intersimple sequence repeats; genetic diversity; ScMATE1 gene; exons; Secale

The objective of this study was to quantify the molecular diversity and to determine the genetic relationships amongSecalespp. and among cultivars ofSecale cerealeusing RAPDs, ISSRs and sequence analysis of six exons ofScMATE1gene.Thirteen ryes (cultivated and wild) were genotyped using 21 RAPD and 16 ISSR primers. A total of 435 markers (242 RAPDsand 193 ISSRs) were obtained, with 293 being polymorphic (146 RAPDs and 147 ISSRs). Two RAPD and nine ISSR primersgenerated more than 80% of polymorphism. The ISSR markers were more polymorphic and informative than RAPDs. Further,69% of the ISSR primers selected achieved at least 70% of DNA polymorphism. The study of six exons of theScMATE1gene also demonstrated a high genetic variability that subsists inSecalegenus. One difference observed in exon 1 sequencesfromS. vaviloviiseems to be correlated with Al sensitivity in this species. The genetic relationships obtained using RAPDs,ISSRs and exons ofScMATE1gene were similar.S. ancestrale

,S. kuprijanoviiandS. cerealewere grouped in the same clusterandS. segetalewas in another cluster.S. vaviloviishowed evidences of not being clearly an isolate species and having greatintraspecific difference

E. SANTOS^{1 2} M. MATOS^{1 2} P. SILVA¹ A. M. FIGUEIRAS³ C. BENITO³ O. PINTO-CARNIDE^{1 4}

1. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Quinta de Prados, 5001-801 Vila Real, Portuga
2. BioISI- Biosystems & Integrative Sciences Institute, University of Lisboa, Faculty of Sciences, Campo Grande, Lisboa, Portugal
3. Departamento de Genética, Facultad de Biologia, Universidad Complutense, C/ José Antonio Novais, 12, 28040 Madrid, Spain
4. CITAB -Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portuga

Manuscript received

20 February 2015

Manuscript revised

10 July 2015

Accepted

3 September 2015

Unedited version published online

9 September 2015

Final version published online

18 April 2016

• 0273-0281-SI.pdf