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    • Keywords

       

      PISTILLATA-like gene; C-terminal domain; flower development; Bambusa oldhamii.

    • Abstract

       

      Bamboo flowering owns many unique characteristics and remains a mystery. To investigate the molecular mechanisms under-lying flower development in bamboo, a petal-identity gene was identified as a PISTILLATA homologue named BoPI from

      Bambusa oldhamii (bamboo family). Expression analysis showed that BoPI was highly expressed in flower organs and gradu-ally increased during flower development stage, suggesting that BoPI played an important role in flower development. Ectopicexpression of BoPI in Arabidopsis caused conversion of sepals to petals. 35S:: BoPI fully rescued the defective petal forma-tion in thepi-1 mutant. BoPI could interact with BoAP3 protein in vitro. These results suggested that BoPI regulated flowerdevelopment of bamboo in a similar way with PI. Besides flower organs, BoPI was also expressed in leaf and branch, whichrevealed that BoPI may involve in leaf and branch development. Similar to other MIKC-type gene, BoPI contained the C-terminal sequence but its function was controversial. Ectopic expression of the C-terminal deletion construct (BoPI-$\delta${C}) in

      Arabidopsis converted sepals to petals; BoPI-$\delta${C} interacted with BoAP3 on yeast two-hybrid assay, just like the full-lengthconstruct. The result implied that the C-terminal sequence may not be absolutely required for organ identity function in thecontext of BoPI.

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