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      https://www.ias.ac.in/article/fulltext/jgen/094/04/0697-0704

    • Keywords

       

      agarwood; conservation; home gardens; genetic diversity; population genetic structure; amplified fragment length polymorphism.

    • Abstract

       

      The endangered Aquilaria malaccensis, is an important plant with high economic values. Characterization of genetic diversity and population structure is receiving tremendous attention for effective conservation of genetic resources. Considering important repositories of biological diversity, the genetic relationships of 127 A. malaccensis accessions from 10 home gardens of three states of northeast India were assessed using amplified fragment length polymorphism (AFLP). Of the 1153 fragments amplified with four AFLP primer combinations, 916 (79.4%) were found to be polymorphic. Polymorphic information content (PIC) and marker index (MI) of each primer combination correlate significantly with the number of genotypes resolved. Overall, a high genetic diversity (avg. 71.85%) was recorded. Further, high gene flow (𝑁m : 3.37), low genetic differentiation (𝐹ST : 0.069) and high within population genetic variation (93%) suggests that most of the genetic diversity is restricted within population. Neighbour joining (NJ), principal coordinate analysis (PCoA) and Bayesian-based STRUCTURE grouped all the accessions in two clusters with significant intermixing between populations, therefore, revealed that two genetically distinct gene pools are operating in the A. malaccensis populations cultivated in home gardens. Based on the various diversity inferences, five diverse populations (JOH, FN, HLF, DHM and ITN) were identified, which can be potentially exploited to develop conservation strategies for A. malaccensis.

    • Author Affiliations

       

      Pradeep Singh1 Akshay Nag1 Rajni Parmar1 Sneha Ghosh2 Brijmohan Singh Bhau2 Ram Kumar Sharma1

      1. Division of Biotechnology, CSIR - Institute of Himalayan Bioresource Technology, P.O. Box 6, Palampur 176 061, India
      2. Division of Medicinal Aromatic and Economic Plants, CSIR - North-East Institute of Science and Technology (CSIR-NEIST), Jorhat 785 006, India
    • Dates

       
  • Journal of Genetics | News

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