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    • Keywords


      bmr-6; cinnamyl alcohol dehydrogenase; lignin biosynthesis; sorghum.

    • Abstract


      Brown midrib mutants in sorghum are associated with reduced lignin content and increased cell wall digestibility. In this study, we characterized a bmr-6 sorghum mutant, which shows reddish pigment in the midrib and stem after the fifth-leaf stage. Compared to wild type, Kalson lignin content of bmr-6 is decreased significantly. We used histological analysis to determine that the mutant exhibited a modified pattern of lignin staining and found an increased polysaccharide content. We cloned BMR-6 gene, a gene encoded a cinnamyl alcohol dehydrogenase (CAD), using a map-based cloning approach. Genetic complementation confirmed that CAD is responsible for the BMR-6 phenotype. BMR-6 gene was expressed in all tested sorghum tissues, with the highest being in midrib and stem. Transient expression assays in Nicotiana benthamiana leaves demonstrated cytomplasmic localization of BMR-6. We found that the expression level of bmr-6 was significantly decreased in the mutant but expression of SbCAD3 and SbCAD5 were significantly increased. Our results indicate that BMR-6 not only affects the distribution of lignin but also the biosynthesis of lignin in sorghum.

    • Author Affiliations


      Jieqin Li1 Lihua Wang1 Qiuwen Zhan1 Yanlong Liu1

      1. College of Agriculture, Anhui Science and Technology University, Fengyang 233100, People’s Republic of China
    • Dates

  • Journal of Genetics | News

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      Posted on July 25, 2019

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