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monosomic alien addition line; disomic alien addition line; linkage group; chromosome; Brassica rapa ssp. pekinensis; B. oleracea var. capitata.

Five monosomic alien addition lines (MAALs) of Brassica rapa ssp. pekinensis – B. oleracea var. capitata were obtained by hybridization and backcrossing between B. rapa ssp. pekinensis (female parent) and B. oleracea var. capitata. The alien linkage groups were identified using 42 B. oleracea var. capitata linkage group-specific markers as B. oleracea linkage groups C2, C3, C6, C7 and C8. Based on the chromosomal karyotype of root tip cells, these five MAALs added individual chromosomes from B. oleracea var. capitata: chr 1 (the longest), chr 2 or 3, chr 5 (small locus of 25S rDNA), chr 7 (satellite-carrying) and chr 9 (the shortest). Five disomic alien addition lines were then generated by selfing their corresponding MAALs.

Ai xia Gu¹ Shu Xing Shen¹ Yan Hua Wang¹ Jian Jun Zhao¹ Shu Xin Xuan¹ Xue Ping Chen¹ Xiao Feng Li¹ Shuang Xia Luo¹ Yu Jing Zhao¹

1. College of Horticulture, Agricultural University of Hebei, No. 289, Lingyusi Road, Baoding 071001, People’s Republic of China

Published

September 2015

Manuscript received

8 November 2014

Manuscript revised

2 March 2015

Accepted

9 March 2015

Unedited version published online

13 March 2015

Early published

11 September 2015