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      https://www.ias.ac.in/article/fulltext/jgen/094/01/0075-0085

    • Keywords

       

      DNA methylation; PPP1R3C gene; colorectal cancer; high glucose; proliferation.

    • Abstract

       

      DNA methylation is an epigenetic event that occurs frequently in colorectal cancer (CRC). Increased glucose level is a strong risk factor for CRC. Protein phosphatase 1 regulatory subunit 3C (PPP1R3C) modulates glycogen metabolism, particularly glycogen synthesis. The aim of this study was to investigate the effect of high glucose levels on DNA methylation of PPP1R3C in CRC. PPP1R3C was significantly hypermethylated in CRC tissues (76/105, 72.38%, 𝑃 < 0.05) and colon cancer cell lines (𝑃 < 0.05). CRC tissues obtained from patients with high glucose levels showed that the methylation of PPP1R3C was lower than in patients who had normal levels of glucose. When DLD-1 cells were cultured under conditions of high glucose, the methylation of PPP1R3C was repressed. The expression of PPP1R3C was inversely related to methylation status. In addition, a promoter luciferase assay showed that the transcriptional activity of PPP1R3C was increased in high glucose culture conditions. The number of cells decreased when PPP1R3C was silenced in DLD-1 cells. These results suggest that PPP1R3C, a novel hypermethylated gene in CRC, may play a critical role in cancer cell growth in association with glucose levels.

    • Author Affiliations

       

      Soo Kyung Lee1 Ji Wook Moon1 Yong Woo Lee1 Jung Ok Lee1 Su Jin Kim1 Nami Kim1 Jin Kim2 Hyeon Soo Kim1 Sun-Hwa Park1

      1. Department of Anatomy, Korea University Anam Hospital, Korea University College of Medicine, 126-1, Anam-dong 5-ga, Seongbuk-gu, Seoul, South Korea
      2. Department of General Surgery, Korea University Anam Hospital, Korea University College of Medicine, 126-1, Anam-dong 5-ga, Seongbuk-gu, Seoul, South Korea
    • Dates

       
  • Journal of Genetics | News

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