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      https://www.ias.ac.in/article/fulltext/jgen/093/01/0035-0041

    • Keywords

       

      gluten; cysteine; coeliac disease; evolution; 𝛾-prolamin; Triticeae.

    • Abstract

       

      The 𝛾-prolamins are important components of seed storage proteins in wheat and other Triticeae species. Here, the 𝛾-prolamin genes from the diploid Triticeae species were systemically characterized. Most of the 𝛾-prolamins (except 75 K 𝛾-secalins) characterized were defined as 𝛾-gliadin-like 𝛾-prolamins, since they shared same characteristic model structure with 𝛾-gliadins. Over one-third of these putatively functional 𝛾-prolamin peptides contained different number of cysteine residues as compared to the eight residues present in 𝛾-gliadins. Sequence polymorphism and linkage disequilibrium analyses showed the conservation of 𝛾-prolamin genes in Triticeae species under evolutionary selection. Phylogenetic analyses indicated that these 𝛾-prolamin genes can not be clearly separated according to their genomic origins, reflecting the conservation of 𝛾-gliadin-like 𝛾-prolamin genes after the divergence of Triticeae species. A screening of coeliac disease (CD) toxic epitopes shows that the 𝛾-prolamins from some other genomes contain much fewer epitopes than those from the A, S (B) and D genomes of wheat. These findings contribute to better understanding of 𝛾-prolamin family in Triticeae and build a ground for breeding less CD-toxic wheat cultivars.

    • Author Affiliations

       

      Peng-Fei Qi1 Cheng-Xing Le1 Zhao Wang1 Yu-Bin Liu2 Qing Chen1 Zhen-Zhen Wei1 Bin-Jie Xu1 Zheng-Yuan Wei1 Shou-Fen Dai1 Yu-Ming Wei1 You-Liang Zheng1 3

      1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, People’s Republic of China
      2. Agricultural Science Research Institute, Xichang, Sichuan 615000, People’s Republic of China
      3. Ministry of Education Key Laboratory for Crop Genetic Resources and Improvement in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, People’s Republic of China
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  • Journal of Genetics | News

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