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    • Keywords


      mutation; recombination; DNA sequence; primary metabolism; trp-3.

    • Abstract


      Fifteen different classically generated and mapped mutations at the tryptophan synthetase locus in Neurospora crassa have been characterized to the level of the primary sequence of the gene. This sequence analysis has demonstrated that intragenic recombination is accurate to order mutations within one open reading frame. While classic genetic analysis correctly ordered the mutations, the position of mutations characterized by gene sequence analysis was more accurate. A leaky mutation was found to have a wild-type primary sequence. The presence of unique polymorphisms in the primary sequence of the trp-3 gene from strain 861 confirms that it has a unique history relative to the other strains studied. Most strains that were previously shown to be immunologically nonreactive with antibody preparations raised against tryptophan synthetase protein were shown to have nonsense mutations. This work defines 14 alleles of the N. crassa trp-3 gene.

    • Author Affiliations


      A. Wiest1 D. Barchers1 2 M. Eaton1 3 R. Henderson1 4 R. Schnittker1 K. Mccluskey1

      1. Fungal Genetics Stock Center, University of Missouri, Kansas City, MO 64110, USA
      2. Truman High School, 3301 S. Noland Road, Independence, MO 64055, USA
      3. Blue Valley North High School, 12200 Lamar Avenue, Overland Park, KS 66209, USA
      4. Blue Springs North High School, 2000 NW Ashton Drive, Blue Springs, MO 64015, USA
    • Dates

    • Supplementary Material

  • Journal of Genetics | News

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      Posted on July 25, 2019

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