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      https://www.ias.ac.in/article/fulltext/jgen/092/03/0363-0368

    • Keywords

       

      carotenoid biosynthesis; carotenoid pathway genes; tomato; lycopene; Pusa Rohini; ripening.

    • Abstract

       

      Fruit ripening process is associated with change in carotenoid profile and accumulation of lycopene in tomato (Solanum lycopersicum L.). In this study, we quantified the 𝛽-carotene and lycopene content at green, breaker and red-ripe stages of fruit ripening in eight tomato genotypes by using high-performance liquid chromatography. Among the genotypes, lycopene content was found highest in Pusa Rohini and lowest in VRT-32-1. To gain further insight into the regulation of lycopene biosynthesis and accumulation during fruit ripening, expression analysis of nine carotenoid pathway-related genes was carried out in the fruits of high lycopene genotype—Pusa Rohini. We found that expression of phytoene synthase and 𝛽-carotene hydroxylase-1 was four and thirty-fold higher, respectively, at breaker stage as compared to red-ripe stage of fruit ripening. Changes in the expression level of these genes were associated with a 40% increase in lycopene content at red-ripe stage as compared with breaker stage. Thus, the results from our study suggest the role of specific carotenoid pathway-related genes in accumulation of high lycopene during the fruit ripening processes.

    • Author Affiliations

       

      Shuchi Smita1 2 Ravi Rajwanshi1 3 Sangram Keshari Lenka1 4 Amit Katiyar1 5 Viswanathan Chinnusamy6 Kailash Chander Bansal1 7

      1. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, New Delhi 110 012, India
      2. Centre for Agricultural Bioinformatics, Indian Agricultural Research Institute Campus, New Delhi 110 012, India
      3. Department of Biotechnology, School of Life Sciences, Assam University, Silchar 788 011, India
      4. Reliance Industries Limited, Mumbai 400 701, India
      5. Indian Council of Medical Research, Headquarter, Ansari Nagar, New Delhi 110 029, India
      6. Division of Plant Physiology, Indian Agricultural Research Institute Campus, New Delhi 110 012, India
      7. National Bureau of Plant Genetic Resources, Indian Agricultural Research Institute Campus, New Delhi 110 012, India

    • Dates

       
      Published
      December 2013
      Manuscript received
      15 March 2013
      Manuscript revised
      5 May 2013
      Accepted
      15 May 2013
      Early published
      23 October 2013

    • Supplementary Material

       

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