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      https://www.ias.ac.in/article/fulltext/jgen/090/01/0103-0109

    • Keywords

       

      DArT; genetic mapping; molecular markers; pigeon pea.

    • Abstract

       

      With an objective to develop a genetic map in pigeon pea (Cajanus spp.), a total of 554 diversity arrays technology (DArT) markers showed polymorphism in a pigeon pea F2 mapping population of 72 progenies derived from an interspecific cross of ICP 28 (Cajanus cajan) and ICPW 94 (Cajanus scarabaeoides). Approximately 13% of markers did not conform to expected segregation ratio. The total number of DArT marker loci segregating in Mendelian manner was 405 with 73.1% ($P \gt 0.001$) of DArT markers having unique segregation patterns. Two groups of genetic maps were generated using DArT markers. While the maternal genetic linkage map had 122 unique DArT maternal marker loci, the paternal genetic linkage map has a total of 172 unique DArT paternal marker loci. The length of these two maps covered 270.0 cM and 451.6 cM, respectively. These are the first genetic linkage maps developed for pigeon pea, and this is the first report of genetic mapping in any grain legume using diversity arrays technology.

    • Author Affiliations

       

      Shi Ying Yang1 2 3 Rachit A. Saxena4 Pawan L. Kulwal5 Gavin J. Ash2 Anuja Dubey4 6 John D. I. Harper2 Hari D. Upadhyaya4 Ragini Gothalwal6 Andrzej Kilian1 Rajeev K. Varshney4 7

      1. Diversity Arrays Technology Pty Limited, 1 Wilf Crane Crescent, Yarralumla, Canberra, ACT 2600, Australia
      2. EH Graham Centre for Agricultural Innovation (NSW Department of Industry and Investment and Charles Sturt University), P. O. Box 588 Wagga Wagga, NSW 2650, Australia
      3. Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, People’s Republic of China
      4. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, India
      5. Mahatma Phule Agricultural University, Rahuri, Ahmednagar 413 722, Maharashtra, India
      6. Department of Biotechnology and Bioinformatics Centre, Barkatullah University, Bhopal 462 026, India
      7. Theme-Comparative and Applied Genomics, Generation Challenge Program (GCP), c/o CIMMYT, Int APDO Postal 6–641, 06600 Mexico DF, Mexico
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    • Supplementary Material

       
  • Journal of Genetics | News

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