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      https://www.ias.ac.in/article/fulltext/jgen/089/01/0009-0019

    • Keywords

       

      AFLP; disease resistance; DNA markers; downy mildew; Papaver somniferum; Peronospora arborescens.

    • Abstract

       

      Downy mildew (DM) caused by Peronospora arborescens, is a serious disease in opium poppy (Papaver somniferum), which has a world-wide spread. The establishment of DM-resistant cultivars appears to be a sustainable way to control the disease. In this paper, we present the results of a study aimed at the identification of amplified fragment length polymorphism (AFLP) markers for DM-resistance in opium poppy. Three opium poppy genotypes (inbred over about 10 years): Pps-1 (DM-resistant), Jawahar-16 (DM-susceptible) and H-9 (DM-susceptible) were crossed in a diallel manner and the F1 progeny along with the parents were subjected to AFLP analysis of chloroplast (cp) and nuclear DNA with seven and nine EcoRI / MseI primer combinations, respectively. cpDNA AFLP analysis identified 24 Pps-1 (DM-resistant)-specific unique fragments that were found to be maternally inherited in both the crosses, Pps-1 × Jawahar-16 and Pps-1 × H-9. In the case of nuclear DNA AFLP analysis, it was found that 17 fragments inherited from Pps-1 were common to the reciprocal crosses of both

      1. Pps-1 and Jawahar-16 as well as

      2. Pps-1 and H-9.

      This is the first molecular investigation on the identification of polymorphism between DM-resistant and DM-susceptible opium poppy genotypes and development of DM-resistant opium poppy genotype-specific AFLP markers. These AFLP markers could be used in future genetic studies for analysis of linkage to the downy mildew resistance trait.

    • Author Affiliations

       

      Mukesh K. Dubey1 2 Ajit K. Shasany3 Om P. Dhawan1 Ashutosh K. Shukla3 Suman P. S. Khanuja3 4

      1. Genetics and Plant Breeding Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, Lucknow 226 015, India
      2. Avesthagen Limited, Discoverer 9th Floor, International Technology Park, Whitefield Road, Bangalore 560 066, India
      3. Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, Lucknow 226 015, India
      4. C41-42, DS, Ramesh Nagar, New Delhi 110 015, India
    • Dates

       
  • Journal of Genetics | News

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