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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jgen/088/02/0227-0232

    • Keywords

       

      DNA markers; genetic mapping; leaf-blade mutant; multifoliate pinna gene; pea; Pisum sativum.

    • Abstract

       

      The multifoliate pinna (mfp) mutation alters the leaf-blade architecture of pea, such that simple tendril pinnae of distal domain are replaced by compound pinna blades of tendrilled leaflets in mfp homozygotes. The MFP locus was mapped with reference to DNA markers using F2 and F2:5 RIL as mapping populations. Among 205 RAPD, 27 ISSR and 35 SSR markers that demonstrated polymorphism between the parents of mapping populations, three RAPD markers were found linked to the MFP locus by bulk segregant analyses on mfp/mfp and MFP/MFP bulks assembled from the F2:5 population. The segregational analysis of mfp and 267 DNA markers on 96 F2 plants allowed placement of 26 DNA markers with reference to MFP on a linkage group. The existence of common markers on reference genetic maps and MFP linkage group developed here showed that MFP is located on linkage group IV of the consensus genetic map of pea.

    • Author Affiliations

       

      Raghvendra Kumar Mishra1 2 Anil Kumar2 Swati Chaudhary1 Sushil Kumar1

      1. National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi 110 067, India
      2. School of Biotechnology, Devi Ahilya University, Indore 452 001, India
    • Dates

       
  • Journal of Genetics | News

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