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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jgen/087/02/0119-0125

    • Keywords

       

      chromosome identification; Crassostrea gigas; fluorescence in situ hybridization (FISH); mollusks; simple sequence repeats (SSRs).

    • Abstract

       

      Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)4, (GT)7 and (TA)10 onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa.

    • Author Affiliations

       

      K. Bouilly1 R. Chaves1 A. Leitão1 2 A. Benabdelmouna3 H. Guedes-Pinto1

      1. Institute for Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, (IBB/CGB-UTAD), 5001-801 Vila Real, Portugal
      2. IPIMAR, Southern Regional Centre of Fisheries Research CRIP-Sul, Avenida 5 de Outubro, 8700-305 Olhão, Portugal
      3. IFREMER, Laboratoire de Génétique et Pathologie, 17390 La Tremblade, France
    • Dates

       
  • Journal of Genetics | News

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