• Homeotic-like modification of stamens to petals is associated with aberrant mitochondrial gene expression in cytoplasmic male sterile OguraBrassica juncea

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      https://www.ias.ac.in/article/fulltext/jgen/085/02/0133-0139

    • Keywords

       

      CMS (Ogu)Brassica juncea; mustard; atp6 ; homeotic floral modification; petaloid anthers

    • Abstract

       

      We have previously reported correction of severe leaf chlorosis in the cytoplasmic male sterile Ogura (also called Ogu)Brassica juncea line carrying Ogura cytoplasm by plastid substitution via protoplast fusion. Two cybrids obtained from the fusion experiment, Og1 and Og2, were green and carried the plastid genome ofB. juncea cv. RLM198. While Og1 displayed normal flower morphology comparable to that of its euplasmicB. juncea counterpart except for sterile anthers, Og2 retained homeotic-like floral modification of stamens to petal-like structures and several other floral deformities observed in the chlorotic (Ogu)B. juncea cv. RLM198 (or OgRLM). With respect to the mitochondrial genome, Og1 showed 81% genetic similarity to the fertile cultivar RLM while Og2 showed 93% similarity to OgRLM. In spite of recombination and rearrangements in the mitochondrial genomes in the cybrids, expression patterns of 10 out of 11 mitochondrial genes were similar in all the three CMS lines; the only exception wasatp6, whose expression was altered. While Og1 showed normalatp6 transcript similar to that in RLM, in Og2 and OgRLM weak expression of a longer transcript was detected. These results suggest that the homeotic-like changes in floral patterning leading to petaloid stamens in Og2 and OgRLM may be associated with aberrant mitochondrial gene expression.

    • Author Affiliations

       

      Gargi Meur1 K. Gaikwad2 S. R. Bhat2 S. Prakash2 P. B. Kirti1

      1. Department of Plant Sciences, University of Hyderabad, Hyderabad - 500 046, India
      2. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi - 110 012, India
    • Dates

       
  • Journal of Genetics | News

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