• Isolation and characterization of stable mutants ofStreptomyces peucetius defective in daunorubicin biosynthesis

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      https://www.ias.ac.in/article/fulltext/jgen/080/01/0031-0038

    • Keywords

       

      Streptomyces peucetius ; daunorubicin; ε-rhodomycinone; NTG mutagenesis

    • Abstract

       

      Daunorubicin and its derivative doxorubicin are antitumour anthracycline antibiotics produced byStreptomyces peucetius. In this study we report isolation of stable mutants ofS. peucetius blocked in different steps of the daunorubicin biosynthesis pathway. Mutants were screened on the basis of colony colour since producer strains are distinctively coloured on agar plates. Different mutants showed accumulation of aklaviketone, ε-rhodomycinone, maggiemycin or 13-dihydrocarminomycin in their culture filtrates. These results indicate that the mutations in these isolates affect steps catalysed bydnrE (mutants SPAK and SPMAG),dnrS (SPFS and SPRHO) anddoxA (SPDHC) gene products.

    • Author Affiliations

       

      K. S. Vetrivel1 K. Dharmalingam1

      1. Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai - 625 021, India
    • Dates

       
  • Journal of Genetics | News

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