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    • Keywords


      genetic suppression; protein-protein interaction; allele specificity; long-range interaction

    • Abstract


      Protein-protein interactions influence many cellular processes and it is increasingly being felt that even a weak and remote interplay between two subunits of a protein or between two proteins in a complex may govern the fate of a particular biochemical pathway. In a bacterial system where the complete genome sequence is available, it is an arduous task to assign function to a large number of proteins. It is possible that many of them are peripherally associated with a cellular event and it is very difficult to probe such interaction. However, mutations in the genes that encode such proteins (primary mutations) are useful in these studies. Isolation of a suppressor or a second-site mutation that restores the phenotype abolished by the primary mutation could be an elegant yet simple way to follow a set of interacting proteins. Such a reversion site need not necessarily be geometrically close to the primary mutation site.

    • Author Affiliations


      Sitaraman Sujatha1 Dipankar Chatterji1

      1. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

  • Journal of Genetics | News

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      Posted on July 25, 2019

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