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      https://www.ias.ac.in/article/fulltext/jgen/079/03/0125-0129

    • Keywords

       

      genetic suppression; protein-protein interaction; allele specificity; long-range interaction

    • Abstract

       

      Protein-protein interactions influence many cellular processes and it is increasingly being felt that even a weak and remote interplay between two subunits of a protein or between two proteins in a complex may govern the fate of a particular biochemical pathway. In a bacterial system where the complete genome sequence is available, it is an arduous task to assign function to a large number of proteins. It is possible that many of them are peripherally associated with a cellular event and it is very difficult to probe such interaction. However, mutations in the genes that encode such proteins (primary mutations) are useful in these studies. Isolation of a suppressor or a second-site mutation that restores the phenotype abolished by the primary mutation could be an elegant yet simple way to follow a set of interacting proteins. Such a reversion site need not necessarily be geometrically close to the primary mutation site.

    • Author Affiliations

       

      Sitaraman Sujatha1 Dipankar Chatterji1

      1. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

       
  • Journal of Genetics | News

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