• Isolation of acdc28 mutation that abrogates the dependence of S phase on completion of M phase of the budding yeast cell cycle

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    • Keywords


      yeast; Cdc28 kinase; checkpoint; cell cycle

    • Abstract


      We have isolated a mutation in the budding yeastSaccharomyces cerevisisae CDC28 gene that allowscdc13 cells, carrying damaged DNA, to continue with the cell division cycle. Whilecdc13 mutant cells are arrested as largebudded cells at the nonpermissive temperature 37‡C, thecdc13 cdc28 double mutant culture showed cells with one or more buds, most of which showed apical growth. The additional buds emerged without the intervening steps of nuclear division and cell separation. We suggest that thecdc28 mutation abrogates a checkpoint function and allows cells with damaged or incompletely replicated DNA an entry to another round of cell cycle and bypasses the mitotic phase of the cell cycle.

    • Author Affiliations


      Santanu Kumar Ghosh1 Pratima Sinha1

      1. Department of Biochemistry, Bose Institute, Calcutta - 700 054, India
    • Dates

  • Journal of Genetics | News

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      Posted on July 25, 2019

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