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    • Keywords


      sex determination; Sxl ; FLP-recombinase; FRT.

    • Abstract


      Drosophila melanogaster females homozygous forflex, an X-linked recessive mutation, do not survive. Hemizygous males are unaffected. Homozygous embryos appear to lack SXL, the product of theSex-lethal (Sxl) gene, apparently as a result of disruption ofSxl splicing. It is known that bothSxl and its somatic splicing regulators [snf andfl(2)d] also function in the development of the female germ line. For this reason, we investigated the role offlex in the germ line by generatingflex/flex clones inflex/+ females. Females carrying such clones in their germ lines do not lay eggs whereas females carryingflex+ eggs lay viable eggs. Additionally, DAPI staining of ovarioles showed that diploid germ cells that are homozygous mutant forflex do not complete oogenesis. These results indicate that theflex+ gene product may be required for the development of the female germ line.

    • Author Affiliations


      Ananya Bhattacharya1 2 S. Sudha1 Swathi Balakrishna1 H. Sharat Chandra1 3

      1. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore - 560012, India
      2. Waksman Institute, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ - 08854-8020, USA
      3. Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore - 560 064, India
    • Dates

  • Journal of Genetics | News

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      Posted on July 25, 2019

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