• Cloning and expression inEscherichia coli of entomotoxic protein gene fromBacillus thuringiensis subspecieskurstaki

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      https://www.ias.ac.in/article/fulltext/jgen/068/03/0147-0160

    • Keywords

       

      Bacillus thuringiensis ; delta-endotoxin; gene cloning and expression; lepidopteran toxin; Spodoptera toxin; parasporal crystal protein; toxin gene

    • Abstract

       

      A plasmid borne larvicidal crystal protein gene from B.thuringiensis subspecieskurstaki was cloned inEscherichia coli using a specific 20-mer oligonucleotide probe. The gene expressed inE. coli at a high level. TransgenicE. coli cells produced large irregular bodies which looked bright under phase contrast microscopy. The phase bright bodies released by sonic disruption of cells could be pelleted by centrifugation. Toxicity trials on the larvae ofSpodoptera litura showed that the pellet was antifeedant and toxic to the larvae. The supernatant was only mildly antifeedant. Even short term feeding of larvae on the toxin delayed the onset of pupation.

    • Author Affiliations

       

      Rakesh Tuli1 Jyoti Saluja1 Nihal K. Notani1

      1. Molecular Biology and Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay - 400 085, India
    • Dates

       
  • Journal of Genetics | News

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