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    • Keywords


      Drosophila ; Sgs-3 ; l(1)npr-1 ; trans-acting; glue; ecdysterone

    • Abstract


      The 2B5 chromosomal locus inDrosophila contains a gene,I(1)npr-1+, whose product required intrans for the expression of the larval salivary gland-specific geneSgs-3. We have addressed the question as to whether this factor acts in a cell-autonomous manner or not. This was made possible by the use of a transformant strain that makes anSgs-3-E. coli β-galactosidase (lacZ) fusion protein, under the control of anSgs-3 promoter, allowing the cellular examination of gene expression by a histochemical assay for enzyme activity. Using genetic methods, larvae that were mosaic for the loss of function mutationl(l)npr-1 were generated. The expression of theSgs-3-lacZ fusion gene was assayed histochemically in such larvae. Our results strongly indicate a cell-autonomous requirement for the product ofl(1)npr-1+. This is in contrast to another factor, the hormone ecdysterone, which is also required forSgs-3 expression, but acts in a non-autonomous manner

    • Author Affiliations


      K. Vijay Raghavan1 2 Carol Mayeda1 Elliot M. Meyerowitz1

      1. Division of Biology, California Institute of Technology, Pasadena, California - 91125, USA
      2. Molecular Biology Unit, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Bombay - 400 005, India
    • Dates

  • Journal of Genetics | News

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      Posted on July 25, 2019

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