• TheInt family of site-specific recombinases: Some thoughts on a general reaction mechanism

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      https://www.ias.ac.in/article/fulltext/jgen/067/01/0029-0036

    • Keywords

       

      Int-family recombinases; FLP protein variants; site-specific recombination

    • Abstract

       

      The FLP recombinase of the yeast 2 micron circle plasmid belongs to theInt family of recombinases. Only three amino acid residues are invariant among members of this family. Functional analyses of FLP protein variants mutated at these three residues suggest their involvement at specific steps of the recombination pathway. We propose that these residues play the same functional role in the mechanism of action of all theInt family recombinases.

    • Author Affiliations

       

      Makkuni Jayaram1

      1. Department of Molecular Biology, Research Institute of Scripps Clinic, 10666 North Torrey Pines Road, La Jolla, CA - 92037, USA
    • Dates

       
  • Journal of Genetics | News

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