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    • Keywords


      Dictyostelium discoideum ; nystatin; pimaricin; azasterol; fenarimol

    • Abstract


      Spontaneous nystatin resistance mutations inDictyostelium discoideum fall into three complementation groups;nys A. nys B andnys C. We demonstrate three methods for rapidly distinguishing mutations in the three complementation groups. In the first methodnys B andnys C mutations are identified by their sensitivity to the sterol biosynthesis inhibitors azasterol A25822B and fenarimol respectively. The second method exploits the differential sensitivities of thenys mutations to the polyene antibiotic pimaricin. In the last method we show thatnys C and non-nys C mutants can be distinguished on the basis of the Lieberman-Burchard color reaction for sterols.

    • Author Affiliations


      Durgadas P Kasbekar1 2 Sanford Madigan2 Eggerton Campbell2 3 Eugene R Katz2

      1. Department of Genetics, Albert Einstein College of Medicine, Bronx, NY - 10461, USA
      2. Department of Microbiology, State University of New York at Stony Brook, Stony Brook, NY - 11794-8621, USA
      3. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY - 10461, USA
    • Dates

  • Journal of Genetics | News

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      Posted on July 25, 2019

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