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      https://www.ias.ac.in/article/fulltext/jgen/066/01/0025-0031

    • Keywords

       

      A. tumefaciens (pTi KanR); tobacco leaf disc infection; genetic transformation; vertical transmission; monohybrid and dihybrid segregations

    • Abstract

       

      A chimeric DNA construction having nopaline synthase promoter, coding sequences of neomycin phosphotransferase gene conferring resistance to antibiotic kanamycin and OCS (octopine synthase) polyadenylation sequences bracketed by T-DNA ends was transferred to tobacco. Leaf discs were infected withA. tumefaciens containing disarmed, cointegrate plasmid pGV3850:: 1103 and allowed to form a callus in the presence of kanamycin. Shoots regenerated from infected leaf discs either through the callus or arising directly were further selected for their ability to root in kanamycin-containing media. Among the nine transgenic plants that were progeny tested, the transferred bacterial gene segregated as monohybrid ratio (3 KanR: 1 Kans) in seven. Segregation data of two plant progenies indicated the presence of two independent loci of KanR DNA insertion (15 KanR: 1 Kans). Back-cross segregation data were consistent with the monohybrid or independent assortment of duplicate factors. Thus in the two cases, a minimum independent integration of two copies of T-DNA each with a KanR marker is inferred.

    • Author Affiliations

       

      Patricia Viegas1 Helena Mathews2 C. R. Bhatia3 N. K. Notani1

      1. Molecular Biology and Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay - 400 085, India
      2. Bio-Organic Division, Bhabha Atomic Research Centre, Trombay, Bombay - 400 085, India
      3. Nuclear Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay - 400 085, India
    • Dates

       
  • Journal of Genetics | News

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