Cytogenetic analysis of the action of carcinogens and tumour inhibitors inDrosophila melanogaster iv. The cell stage during spermatogenesis and the induction of intra and inter-genic mutations by 2:4:6-tri(ethyleneimino)-1: 3:5-triazine - With seven text-figures
The mutagenic response of the various stages of spermatogenesis to the action of 2:4:6-tri(ethyleneimino)-l:3:5-triazine has been investigated by the brood technique. The treated male was mated to a succession of virgin females at set intervals, and the mutation rate was scored separately in each brood. The mutation rate/brood relationship for the same genetic property varied in the different experiments. It was found to depend on the structure of the testis at the time of treatment and the gross morphological changes induced in it by the mutagen. Another controlling factor is the way the male germ line is fractionated among the broods, which varies with the rate of germ-cell differentiation and the brood interval. To reduce variability between experiments a standard technique was adopted. The treated males were of the same strain, age and size, were reared under the same culture conditions and their progeny was fractionated at fixed intervals.
Volume 100, 2021
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