• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/133/0089

    • Keywords

       

      Photosensitizer; heterobimetallic complexes; photocytotoxicity; autophagy.

    • Abstract

       

      The heterobimetallic Ru(II)-Pt(II) polypyridyl complexes [Ru(bpy)2(BPIMBp)PtCl2]2+ (3) and [Ru(phen)2(BPIMBp)PtCl2]2+ (4) with their parent complexes [Ru(bpy)2BPIMBp]2+(1) and [Ru(phen)2-BPIMBp]2+ (2) possessing bridging ligand (BPIMBp = 1,4ʹ-Bis-{(2-pyridin-2-yl)-1H-imidazol-1-yl)methyl}-1,1ʹ-biphenyl) were used for photocytotoxicity against MCF-7 cells.The parent ruthenium complexes(complexes 1-2) exhibited a negligible increase in viscosity of DNA while heterobimetallic Ru(II)-Pt(II)system exhibited a decrease in viscosity of DNA, indicating covalent interaction (through cis-PtCl2 unit). TheRu(II)-Pt(II) system co-precipitated with CT-DNA confirmed the covalent binding. In electrophoreticmobility studies, the interaction of Ru(II)-Pt(II) system with plasmid DNA led to retardation of negativesupercoiled form, followed by positive supercoiled migration in the dark that indicated the ability to formcovalent adducts with DNA similar to cisplatin. The complexes 1-4 showed enhanced photocleavage ofplasmid DNA on blue light (~450 nm) irradiation. In a cell-based study, all the complexes exhibitedphotoactivated cytotoxicity in MCF-7 cancer cells when exposed to visible light of ~450 nm as compared tolow toxicity in absence of irradiation. Additionally, the complexes containing platinum showed induction ofautophagy in GFP-LC3 expressing MCF-7 cells. Overall our study shows that the inclusion of photosensitizerRu(II) polypyridyl complexes to cis-PtCl2 moiety improves anticancer properties of complexes.

    • Graphical Abstract

       

      The effect of Ru(II)-Pt(II) polypyridyl complexes as photosensitizers was studied against MCF-7 cells. The complexes are negligibly toxic to MCF-7 cells in the dark while highly cytotoxic towards MCF-7 cells on irradiation of blue light (~450 nm). Therefore, the complexes could be potential candidates for Photodynamic Therapy.

    • Author Affiliations

       

      SUSHMA SINGH1 2 MOKSHADA VARMA3 BHUPENDRA SHRAVAGE4 PRASAD KULKARNI3 AVINASH KUMBHAR1

      1. Department of Chemistry, Savitribai Phule Pune University, Pune 411007, India
      2. Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
      3. Bioprospecting group, Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, India
      4. Developmental Biology Group, Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, India
    • Dates

       
  • Journal of Chemical Sciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2021-2022 Indian Academy of Sciences, Bengaluru.