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      https://www.ias.ac.in/article/fulltext/jcsc/133/0044

    • Keywords

       

      (+)-Epicatechin; Bovine serum albumin; UV-vis Spectroscopy; Cyclic voltammetry; Square wave voltammetry; Interactions.

    • Abstract

       

      The mechanism of the electrochemical oxidation of (+)-epicatechin (epiCAT) in a hydro-alcoholicmedium, at a glassy carbon electrode, was studied using cyclic and square wave voltammetrictechniques and at different pHs. It proceeds in a cascade mechanism related to the two resorcinol hydroxylgroups and the other three hydroxyl groups in epiCAT which present electroactivity and is pH-dependent. Theoxidation of the 3′,4′-dihydroxyl moiety, occurs first at a very low positive potential, and it is a two-electron/two proton reversible reaction. The product obtained from the oxidation of the 3′,4′-dihydroxyl moiety thenundergoes a chemical reaction followed by an electrochemical process to give an electroinactive product. Theproposed mechanism is an ECE type mechanism. After the addition of bovine serum albumin (BSA) toepiCAT solution, the oxidation peak currents decreased with no peak potential shift and no new peaksappeared. The diffusion coefficients of both free and bound epiCAT were estimated from the cyclicvoltammetry data (Df = 2.37 9 10-10 cm2s-1 and Db = 6.28 9 10-11 cm2s-1). An epiCAT-BSA complex isformed with binding constant Kapp = 1.8 × 104, calculated from UV-vis spectroscopy data.

    • Graphical Abstract

       

      The oxidation process of (+)-epicatechin (I to I’ and II) in a hydro-alcoholic medium at a glassy carbon electrode follows an ECE type mechanism.

    • Author Affiliations

       

      JULES-BLAISE MABOU LEUNA1 2 MARTIN PENGOU2 3 FRANCIS MERLIN MELATAGUIA TCHIENO3 4 SERGEOT DELOR KUNGO SOP2 ACHILLE NASSI1 5 CHARLES PE´GUY NANSEU-NJIKI2 EMMANUEL NGAMENI2

      1. Laboratoire de Chimie Mine´rale, Faculte´ des Sciences, Universite´ de Douala, B.P. 24157 Douala, Cameroun
      2. Laboratoire de Chimie Analytique, Faculte´ des Sciences, Universite´ de Yaounde´ 1, B.P. 812 Yaounde´, Cameroun
      3. De´partement de Chimie, Faculte´ des Sciences, Universite´ de Maroua, B.P. 814 Maroua, Cameroun
      4. De´partement de Chimie, Faculte´ des Sciences, Universite´ de Dschang, B.P. 67 Dschang, Cameroun
      5. De´partement de Chimie, Faculte´ des Sciences, Universite´ de Sherbrooke, 2500 Boulevard de l’Universite´, Sherbrooke J1K 2R1, Canada
    • Dates

       
  • Journal of Chemical Sciences | News

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