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      https://www.ias.ac.in/article/fulltext/jcsc/133/0042

    • Keywords

       

      Oxovanadium (IV) complexes; Singlet oxygen (1O2); Photo-cytotoxicity in A549 cells; Intracellular ROS generation; Apoptosis.

    • Abstract

       

      Four new ternary Oxovanadium(IV) complexes, [VO(L)(B1-4)](acac), where, L = (E)-2- (2-hydroxybenzylideneamino)-5-guanidinopentanoic acid (Argininesalisylidene) and B were 1H-imidazo-[4,5-f][1,10]-phenanthroline (B1) (1), 2-phenyl-1H-imidazo [4,5-f][1,10] phenanthroline (B2) (2), 2-(naphthalen-1-yl)-1H-imidazo [4,5-f][1,10] phenanthroline (B3) (3) or 1-(pyren-2-yl)-1H-imidazo[4,5-f][1,10] phenanthroline(B4) (4) were synthesized, characterized and photocytotoxicity in A549 cell were studied in dark andvisible light (400–700 nm, 10 J cm-2). Photochemistry of the complexes (1-4) related to the generation of singlet oxygen (1O2) was studied in detail. The presence of triplet excited state in the complexes was probedfrom perylene assay. Photo-activated generation of singlet oxygen from the complexes was probed by DPBF assay and the degree of photo-cytotoxicity of the complexes was related to their ability in photo-activated 1O2 generation. Complexes 4 exhibited remarkable photocytotoxicity in A549 cells with photo index (PI) ~ 16 invisible light (400–700 nm, 10 J cm-2) (IC50, 3.81 lμM), while complexes were almost less-toxic in dark (IC50,59.7 lμM). Intracellular ROS generation was studied from 2Ꞌ,7Ꞌ-dichlorofluorescein diacetate (DCFH-DA).Annexin V-FITC/PI assay suggested apoptotic nature of cell death. Overall, the remarkable photocytotoxicefficacy of the new Oxovanadium(IV) complexes (1-4) making them the potential photochemotherapeutic agents in lieu of porphyrin-based PDT agents.

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    • Author Affiliations

       

      BANDANA SANASAM1 KAUSAR RAZA MD2 DULAL MUSIB1 MITHUN ROY1

      1. Department of Chemistry, National Institute of Technology, Manipur, Langol, 795004 Imphal, Manipur, India
      2. Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore 560012, India
    • Dates

       
  • Journal of Chemical Sciences | News

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