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    • Probing the interaction between human serum albumin and the sodium dodecyl sulphate with fluorescence correlation spectroscopy

      VAISHALI SAMANT ARGHYA DEY G NARESH PATWARI

      Volume 132 Published: 18 August 2020 Article ID 0109

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/132/0109

    • Keywords

       

      Fluorescence Correlation Spectroscopy; Protein-Surfactant Interaction; EIS; human serum albumin;

    • Abstract

       

      The denaturation of human serum albumin (HSA) upon interaction with the surfactant sodium dodecyl sulphate (SDS) was examined by measuring the diffusion time of fluorophore (RITC) tagged HSA under near single-molecule conditions using fluorescence correlation spectroscopy. The diffusion time showsfour distinct regions as a function of SDS concentration, which corresponds to (I) opening of the tertiary structure, (II) non-specific SDS aggregation, (III) opening of the secondary structure, and (IV) aggregation of SDS around the secondary structure. Diffusion time increases from 383 ls for the free protein to 1002 ls forthe SDS bound protein, which leads to an effective increase in the hydrodynamic radius by a factor of about 2.6

    • Author Affiliations

       

      VAISHALI SAMANT1 ARGHYA DEY G NARESH PATWARI

      1. Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra 400 076, India

    • Dates

       
      Published
      18 August 2020
      Manuscript received
      1 April 2020
      Manuscript revised
      10 June 2020
      Accepted
      15 June 2020

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