• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/132/0103

    • Keywords

       

      Antioxidant; ferulic acid; b-lactoglobulin; aggregation

    • Abstract

       

      Amyloids, a well-ordered b-sheet-enriched structural network, can be broadly defined as insoluble protein aggregates that are linked to a wide variety of diseases including systemic amyloidosis and some neurodegenerative disorders. Ferulic acid (FA), a phenolic acid, abundant in antioxidant and efficientpharmaceutical has beneficial effects against several ailments. Based on this, we have investigated the protective role of FA on amyloid formation of bovine b-lactoglobulin (b-lg), a model globular protein. Using a set of in vitro biophysical methods, such as UV-Vis spectroscopy, fluorescence, circular dichroism,transmission electron microscopy, etc., our research group has concluded that FA significantly inhibits the heat-induced amyloid formation of b-lg and this inhibitory effect is dose-dependent. Exposed surface hydrophobicity of b-lg amyloid fibrils decreased significantly in the presence of FA. Docking study revealed that ionic and hydrogen bonding interactions between FA and b-lg prevented protein conformational changes leading to fibrillation. We anticipate that our finding would give an insight into the protein aggregation inhibited by the antioxidant compound, FA and pave the way for finding and developing other new small molecules (protein misfolding inhibitors) that give similar result against amyloid fibril formation and its allied neurodegenerative disorders.

    • Author Affiliations

       

      SAMPA PAL1 SANHITA MAITY1 SUBRATA SARDAR1 SHAHNAZ BEGUM1 RAMKRISHNA DALUI1 HASAN PARVEJ1 KAUSHIK BERA2 ANIRBAN PRADHAN3 NAYIM SEPAY1 SWARNALI PAUL1 UMESH CHANDRA HALDER1

      1. Organic Chemistry Section, Department of Chemistry, Jadavpur University, Kolkata 700 032, India
      2. Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Burdwan 713 104, India
      3. Director’s Research Unit (DRU), Indian Association for the Cultivation of Science, Kolkata 700 032, India
    • Dates

       
  • Journal of Chemical Sciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2021-2022 Indian Academy of Sciences, Bengaluru.