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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/131/04/0028

    • Keywords

       

      Enzymatic biosensor; 1-aminopyrene; reduced graphene oxides; hydrogen peroxide; horseradish peroxidase.

    • Abstract

       

      An electrochemical sensor for hydrogen peroxide (H2O2) detection was established using 1-aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO) modified glassy carbon electrode. The AP can combine with HRP via a covalent bond and combine withrGO via π-π conjugation. The covalent bond linkages can prevent the enzymes leaching of the enzymes and the π-π conjugate combination can facilitate electrons transfer, which plays synergistic effects to improve the performance of this sensor. The electrochemical activity of the HRP-AP/rGO was described by cyclic voltammetry, alternating current impedance and amperometric techniques. Under the optimal conditions, the biosensor showed a wide linear range from 1.5 μM to 28.5 μM and a low detection limit of 0.5 μM with good stability and high selectivity, confirming that the sensor is well-suited for the detection of H2O2 during biological processes.

    • Author Affiliations

       

      NA ZOU1 2 XIANYONG WEI1 3 ZHIMIN ZONG1 XIN LI2 ZHAOXIA WANG2 XUELIANG WANG2

      1. Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining and Technology, Xuzhou 221116, Jiangsu, China
      2. School of Chemistry and Chemical Engineering, Heze University, Heze 274015, Shandong, China
      3. State Key Laboratory of High-Efficiency Coal Utilization and Green Chemical Engineering, Ningxia University, Yinchuan 750021, Ningxia, China
    • Dates

       
  • Journal of Chemical Sciences | News

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