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      https://www.ias.ac.in/article/fulltext/jcsc/130/10/0133

    • Keywords

       

      Gold nanoparticles; porphyrin; MTT assay; singlet oxygen; apoptosis.

    • Abstract

       

      We describe the synthesis and characterization of a thiol-functionalized porphyrin derivative 2 and its gold nanoparticle conjugates. The porphyrin 2 exhibited its characteristic intense Soret absorption at 420 nm with a molar extinction coefficient value of 3.6 × 10 5 M−1cm−1 and good fluorescence in the region of 650–660 nm. The porphyrin-capped gold nanoparticles (POPNPs) were synthesized from the citrate-capped gold nanoparticles by the ligand exchange method and characterized by spectroscopic and morphological analyses(UV–Vis, DLS and TEM). The broadening of the absorption spectrum and quenching of the fluorescence intensity for the porphyrin gold nanoconjugates suggest efficient incorporation of the porphyrin moiety onto the gold surface. The results of DLS and TEM analyses indicate that the nanoconjugate POPNPs are uniformly spherical in shape with a size of ca. 25±5 nm and exhibits a negative zeta potential value of −16.0±2 mV. The singlet oxygen generation efficiency of the porphyrin 2 and POPNPs was calculated and are found to be ca. 0.53 ±0.02 and 0.43 ± 0.03, respectively. The in vitro photobiological studies revealed that POPNPs exhibited enhanced photodynamic activity compared to their parent porphyrin derivative 2 with an IC50 value of 5 μM in MDA MB 231 cell lines. The mechanism of the cell destruction was studied by Annexin-FITC and confirmedthrough TMRM assay. We observed the increase in the percentage of cell population corresponding to the late apoptotic stage ca. 37.7% and 51.2% for 5 and 10 μM of POPNPs, respectively, thereby demonstrating their apoptotic-mediated cell destruction and use in PDT applications

    • Author Affiliations

       

      ALBISH K PAUL1 DHANYA T JAYARAM1 P S SANEESH BABU2 NAGAPPANPILLAI ADARSH1 SHAMEEL THURAKKAL1 3 ASHA S NAIR2 DANABOYINA RAMAIAH4

      1. Photosciences and Photonics Section, Chemical Sciences and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695 019, Kerala, India
      2. Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695 014, Kerala, India
      3. Academy of Scientific and Innovative Research (AcSIR), CSIR-NIIST Campus, Thiruvananthapuram 695 019, Kerala, India
      4. CSIR-North East Institute of Science and Technology, Jorhat 785 006, Assam, India
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  • Journal of Chemical Sciences | News

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