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    • Indicators for suicide substrate inactivation: A kinetic investigation

      SHARMISTHA DHATT

      REGULAR ARTICLE Volume 129 Issue 12 December 2017 pp 1921-1928

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/129/12/1921-1928

    • Keywords

       

      Suicide inhibitor; mechanism-based inhibitor; drug specificity; fractional-life; enzyme inactivation.

    • Abstract

       

      Suicide substrate kinetic pathway and a proposed set of indicators, some theoretical and a few practical ones, that can decisively conclude enzyme inactivation are considered. Steady-state approximation is assumed not only when a catalytic amount of enzyme is used but also for any substrate-enzyme ratio. In each situation, adequacy of the approximation is numerically tested. An equation for the rate of inactivation of the enzyme has been derived and employed under suitable approximations to estimate various indicators.Progressive curves for such indicators via numerical simulation are shown that decide impromptu inactivation too. The scheme though is a traditional one, the findings are novel.

    • Author Affiliations

       

      SHARMISTHA DHATT

      1. Dates

         
        Published
        12 December 2017
        Manuscript received
        20 August 2017
        Manuscript revised
        12 October 2017
        Accepted
        28 October 2017
        Final version published online
        20 November 2017

      2. Supplementary Material

         

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