• Indicators for suicide substrate inactivation: A kinetic investigation

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    • Keywords


      Suicide inhibitor; mechanism-based inhibitor; drug specificity; fractional-life; enzyme inactivation.

    • Abstract


      Suicide substrate kinetic pathway and a proposed set of indicators, some theoretical and a few practical ones, that can decisively conclude enzyme inactivation are considered. Steady-state approximation is assumed not only when a catalytic amount of enzyme is used but also for any substrate-enzyme ratio. In each situation, adequacy of the approximation is numerically tested. An equation for the rate of inactivation of the enzyme has been derived and employed under suitable approximations to estimate various indicators.Progressive curves for such indicators via numerical simulation are shown that decide impromptu inactivation too. The scheme though is a traditional one, the findings are novel.

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