• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/129/07/0841-0847

    • Keywords

       

      Cytochrome c; gold nanoclusters; fluorescence correlation spectroscopy; conformational dynamics.

    • Abstract

       

      Size and structure of cytochrome c (Cyt C) bound to gold nano-clusters (AuNC) were studied using fluorescence correlation spectroscopy (FCS) and circular dichroism (CD) spectroscopy. The CD spectra of Cyt C indicate that the ellipticity is almost completely lost on binding to AuNC which indicates unfolding.Addition of ethanol causes partial restoration of ellipticity and hence, structure of Cyt C. FCS data indicate that size (hydrodynamic radius, rH) of free Cyt C is 17Å which increases to 24Å on binding to AuNC. This too suggests unfolding of Cyt C upon binding to AuNCs. Both the size and conformational relaxation time of Cyt C bound to AuNC vary non-monotonically with increase in ethanol content.

    • Author Affiliations

       

      CATHERINE GHOSH1 M D ASIF AMIN1 BIMAN JANA1 KANKAN BHATTACHARYYA1 2

      1. Department of Physical Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, West Bengal, 700 032, India
      2. Department of Chemistry, Indian Institute of Science Education & Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal, Madhya Pradesh 462 066, India
    • Dates

       
  • Journal of Chemical Sciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.