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    • Development of an electrochemical DNA biosensor for detection of specific Mycobacterium tuberculosis sequence based on poly(L-glutamic acid) modified electrode

      MERVE YESIL SONER DONMEZ FATMA ARSLAN

      Regular Article Volume 128 Issue 11 November 2016 pp 1823-1829

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/128/11/1823-1829

    • Keywords

       

      Poly(L-glutamic acid); DNA sensor; Mycobacteriumtuberculosis.

    • Abstract

       

      An electrochemical DNA biosensor was developed by avidin-biotin interaction of a biotinylated probe and avidin-attached, poly(L-glutamic) acid coated pencil graphite electrode (PGA/PGE) for detection of specific Mycobacterium tuberculosis DNA sequence. The discrimination of fully complementary hybridization and mismatch hybridization was carried out by electrochemical reduction current of Meldola’s Blue (MDB) in square wave voltammetry (SWV). The calibration graph of the DNA biosensor was linear between 1.5–12.5 nM and the detection limit was calculated as 1.3 nM. The proposed biosensor successfully discriminated short andlong oligonucleotides related to DNA sequence of Mycobacterium tuberculosis in optimal condition.

    • Author Affiliations

       

      MERVE YESIL1 SONER DONMEZ2 FATMA ARSLAN1

      1. Department of Chemistry, Faculty of Science, Gazi University, 06500, Ankara, Turkey
      2. Bucak School of Health, Mehmet Akif Ersoy University, 15300, Bucak, Burdur, Turkey

    • Dates

       
      Published
      2 November 2016
      Manuscript received
      19 April 2016
      Manuscript revised
      4 August 2016
      Accepted
      9 August 2016

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