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    • Keywords


      Bimetallic nanoparticles; Nanocomposite; DNA plasmid; Biosensor.

    • Abstract


      A new label-free electrochemical DNA biosensor is presented based on carbon paste electrode (CPE) modified with gold (Au) and platinum (Pt) nanoparticles to prepare the bimetallic nanocomposite electrode. The proposed sensor was made by immobilization of 15-mer single stranded oligonucleotide probe related to p53 gene for detection of DNA plasmid samples. The hybridization detection relied on the alternation in the guanine oxidation signal following hybridization of the probe with complementary genomic DNA.The technique of differential pulse voltammetry (DPV) was used for monitoring guanine oxidation. To optimize the performance of the modified CPE, different electrodes were prepared in various percentages of Au and Pt nanoparticles. The modified electrode containing 15% Au/Pt bimetallic nanoparticles (15% Au/Pt-MCPE) was selected as the best working electrode. The selectivity of the sensor was investigated using plasmid samples containing non-complementary oligonucleotides. The detection limit of the biosensor was studied and calculated to be 53.10 pg 𝜇L−1.

    • Author Affiliations


      Ezat Hamidi-Asl1 Jahan-Bakhsh Raoof1 Nahid Naghizadeh1 Simin Sharifi2 Mohammad Saeid Hejazi2 3

      1. Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
      2. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
      3. Drug Applied Research Center and Pharmaceutical Nanotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
    • Dates

  • Journal of Chemical Sciences | News

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