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      https://www.ias.ac.in/article/fulltext/jcsc/127/04/0609-0618

    • Keywords

       

      Iron; polypyridyl bases; DNA binding; photocytotoxicity; apoptosis.

    • Abstract

       

      Iron(II) complexes of polypyridyl ligands (B), viz. [Fe(B)2]Cl2 (1 and 2) of N, N, N-donor 2-(2-pyridyl)-1,10-phenanthroline (pyphen in 1) and 3-(pyridin-2-yl)dipyrido[3,2-a:2’,3’-c]phenazine (pydppz in 2), are prepared and characterized. They are 1:2 electrolytes in aqueous DMF. The diamagnetic complexes exhibit metal to ligand charge transfer band near 570 nm in DMF. The complexes are avid binders to calf thymus DNA giving binding constant (𝐾b) values of ∼106 M−1 suggesting significant intercalative DNA binding of the complexes due to presence of planar phenanthroline bases. Complex 2 exhibits significant photocytotoxicity in immortalized human keratinocyte cells HaCaT and breast cancer cell line MCF-7 giving IC50 values of 0.08 and 13 𝜇M in visible light (400–700 nm). Complex 2 shows only minor dark toxicity in HaCaT cells but is non-toxic in dark in MCF-7 cancer cells. The light-induced cellular damage follows apoptotic pathway on generation of reactive oxygen species as evidenced from the dichlorofluorescein diacetate (DCFDA) assay.

    • Author Affiliations

       

      Aditya Garai1 Uttara Basu1 Ila Pant2 Paturu Kondaiah2 Akhil R Chakravarty1

      1. Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore 560012, India
      2. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India
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    • Supplementary Material

       
  • Journal of Chemical Sciences | News

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