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DNA binding and cleavage activity by a mononuclear iron(II)Schiff base complex: Synthesis and structural characterization
Abhijit Pal Bhaskar Biswas Merry Mitra Subramaniyam Rajalakshmi Chandra Shekhar Purohit Soumitra Hazra Gopinatha Suresh Kumar Balachandran Unni Nair Rajarshi Ghosh
Volume 125 Issue 5 September 2013 pp 1161-1168
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https://www.ias.ac.in/article/fulltext/jcsc/125/05/1161-1168 -
Keywords
Iron(II) ;Schiff base ;X-ray structure ;DNA binding ;DNA cleavage. -
Abstract
Synthesis and characterization of a mononuclear Fe(II) compound [Fe(L)](ClO4)2 (1) [L = N-(1-pyridin-2-yl-phenylidene)-N'-[2-({2-[(1-pyridin-2-ylphenylidene)amino]ethyl}amino)ethyl] ethane-1,2-diamine] (1) is reported. 1 crystallizes in P-1 space group with a = 11.9241(3) Å, b = 12.1994(3) Å and c = 13.0622(4) Å. The binding property of the complex with DNA has been investigated using absorption and emission studies, thermal melting, viscosity experiments and circular dichroism studies. The binding constant ($K_b$) and the linear Stern-Volmer quenching constant ($K_{\text{sv}}$) of the complex have been determined as $3.5 \times 10^3$M-1 and $2.73 \times 10^4$M-1, respectively. Spectroscopic and hydrodynamic investigations revealed intercalative mode of binding of 1 with DNA. 1 is also found to induce oxidative cleavage of the supercoiled pUC 18 DNA to its nicked circular form in a concentration dependent manner.
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Author Affiliations
Abhijit Pal1 Bhaskar Biswas1 2 Merry Mitra1 Subramaniyam Rajalakshmi3 Chandra Shekhar Purohit4 Soumitra Hazra5 Gopinatha Suresh Kumar5 Balachandran Unni Nair3 Rajarshi Ghosh1
- Department of Chemistry, The University of Burdwan, Burdwan 713 104, India
- Department of Chemistry, Raghunathpur College, Purulia 723 133, India
- Chemical Laboratory, CSIR-Central Leather Research Institute, Adayar, Chennai 600 020, India
- Department of Chemical Sciences, National Institute of Science Education and Research, Bhubaneswar 751 005, India
- Biophysical Chemistry Laboratory, Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India
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Dates
- Published
- September 2013
- Manuscript received
- 21 January 2013
- Manuscript revised
- 9 May 2013
- Accepted
- 24 June 2013
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Supplementary Material
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Journal of Chemical Sciences
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