• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/122/06/0911-0917

    • Keywords

       

      Single-stranded DNA; genosensor; self-assembled monolayer; gold functionalized electrode.

    • Abstract

       

      The process of immobilization of biological molecules is one of the most important steps in the construction of a biosensor. In the case of DNA, the way it exposes its bases can result in electrochemical signals to acceptable levels. The use of self-assembled monolayer that allows a connection to the gold thiol group and DNA binding to an aldehydic ligand resulted in the possibility of determining DNA hybridization. Immobilized single strand of DNA (ssDNA) from calf thymus pre-formed from alkanethiol film was formed by incubating a solution of 2-aminoethanothiol (Cys) followed by glutaraldehyde (Glu). Cyclic voltammetry (CV) was used to characterize the self-assembled monolayer on the gold electrode and, also, to study the immobilization of ssDNA probe and hybridization with the complementary sequence (target ssDNA). The ssDNA probe presents a well-defined oxidation peak at +0.158 V. When the hybridization occurs, this peak disappears which confirms the efficacy of the annealing and the DNA double helix performing without the presence of electroactive indicators. The use of SAM resulted in a stable immobilization of the ssDNA probe, enabling the hybridization detection without labels. This study represents a promising approach for molecular biosensor with sensible and reproducible results.

    • Author Affiliations

       

      Mízia M S Silva1 Igor T Cavalcanti1 M Fátima Barroso2 M Goreti F Sales2 Rosa Fireman Dutra1

      1. Laboratório de Pesquisa em Diagnóstico-LAPED, Universidade de Pernambuco, Rua Arnóbio Marques, 310, Santo Amaro, 50100060, Recife-PE, Brazil
      2. Requimte/Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida 431, 4200072, Porto, Portugal
    • Dates

       
  • Journal of Chemical Sciences | News

© 2021-2022 Indian Academy of Sciences, Bengaluru.