In this paper, a novel disposable immunosensor for the detection of 𝛼-fetoprotein (AFP) based on the Indium tin oxide (ITO) modified by the sequential electro-deposition of the nanogold particles (nano-Au) and prussian blue (PB) is described. The ITO is employed to reduce the cost, instead of expensive gold electrode, glassy carbon electrode or platinum electrode. The layer-by-layer (LBL) electro-deposition of the nano-Au, PB is used for blocking the possible leakage from the substrate electrode surface and to prevent shedding of composite membrane. Under optimal conditions, the proposed immunosensor displays a broad linear response to AFP, the working range being 0.25 to 300.0 ng mL^-1 with a detection limit of 0.04 ng mL^-1. The studied immunosensor exhibits high sensitivity, fast analytical time and good stability. The proposed methodology is potentially attractive for clinical immunoassays.