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      https://www.ias.ac.in/article/fulltext/jcsc/121/06/1069-1076

    • Keywords

       

      Indium tin oxide; nanogold particles (nano-Au); 𝛼-fetoprotein; layer-by-layer; electrodeposition.

    • Abstract

       

      In this paper, a novel disposable immunosensor for the detection of 𝛼-fetoprotein (AFP) based on the Indium tin oxide (ITO) modified by the sequential electro-deposition of the nanogold particles (nano-Au) and prussian blue (PB) is described. The ITO is employed to reduce the cost, instead of expensive gold electrode, glassy carbon electrode or platinum electrode. The layer-by-layer (LBL) electro-deposition of the nano-Au, PB is used for blocking the possible leakage from the substrate electrode surface and to prevent shedding of composite membrane. Under optimal conditions, the proposed immunosensor displays a broad linear response to AFP, the working range being 0.25 to 300.0 ng mL-1 with a detection limit of 0.04 ng mL-1. The studied immunosensor exhibits high sensitivity, fast analytical time and good stability. The proposed methodology is potentially attractive for clinical immunoassays.

    • Author Affiliations

       

      Yan Li1 Wen-Bin Liang2 Li-Chao Fang1 Hui Huang1 Jun Deng1 Jun-Song Zheng1

      1. Department of Clinical Laboratory Science, Third Military Medical University, Gaotanyan Street 30, Shapingba District, Chongqing 400038, P R China
      2. Department of Clinical Biochemistry, Laboratory Sciences, Third Military Medical University, Gaotanyan Street 30, Shapingba District, Chongqing 400038, P R China
    • Dates

       
  • Journal of Chemical Sciences | News

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