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    • Keywords


      NMR; 15N and 13C labelled protein; resonance assignment; band-selective pulse; check points; HN(C)N; HN(C)N-ST.

    • Abstract


      We describe here a simple modification of the HN(C)N experiment for the generation of serine/threonine check points in the three-dimensional experiment. The various `triplet of residue’ specific peak patterns in the spectra are documented for ease of analysis and sequential backbone resonance assignment. The performance of this experiment, referred to as HN(C)N-ST, is demonstrated using two proteins, one properly folded and the other completely denatured. It is noteworthy that, even in the denatured protein, where spectral dispersions are rather poor, about 90% of the sequential connectivities through the chain could be established from this single experiment. This would have great implications for structural genomics efforts.

    • Author Affiliations


      Dinesh Kumar1 Jeetender Chugh1 Ramakrishna V Hosur1

      1. Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005
    • Dates

  • Journal of Chemical Sciences | News

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