The interaction of Cu2+ ion with the redox centres of xanthine oxidase (XO) has been investigated using optical difference spectroscopic measurements. Anaerobic enzyme-reduction experiments using controlled and excess substrates (xanthine and NADH) have been performed to investigate the perturbation of XO active sites in the presence of Cu2+ ions. The results indicate an overall alteration in the redox affinities (i.e. affinity to accept electrons) of the active sites of XO by Cu2+ ion.
Volume 134, 2022
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