Ruthenium(III)-edta type complexes for DNA-metallation
Interactions of [RuIII(edta)(H2O)]− (edta=ethylenediaminetetraacetate) with DNA related molecules L (L=DNA-bases, nucleosides, nucleotides and single strand calf-thymus DNA) were studied by spectrophotometric, electrochemical and kinetic methods. The spectral features of substituted product [RuIII(edta)L]− complexes were characterized by a strong ligand-to-metal charge transfer band in the UV region (293–310 nm). However, a blue colour is developed (λmax=582 nm) at highpH (>7.0) for L=GMP (guanosine-5′-monophosphate) Dissociation of N1-H proton of purine portion of coordinated GMP is suggested to promote ligand-to-metal charge band at lower energy (visible) region. The E1/2 values corresponding to RuIII/RuII redox couple for [RuIII(edta)L)− complexes are in the range −0.28 to −0.12 V (vs SCE). Formation kinetics of [RuIII(edta)L]− was studied using stopped-flow methods. Comparing the reactivity of [RuIII(edta)H2O)]− with L in terms of spectral, electrochemical and kinetic data, it is proposed that binding of DNA with Ru(III)-edta complex takes place through adenine base unit.
Volume 134, 2022
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