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    • Keywords


      Collagen aggregates; collagen types; cell; animal tissues; biological function

    • Abstract


      The ultrastructure of animal tissues has a graded distribution that is reminiscent of the reconstituted aggregate structures from isolated type I collagen, type V collagen, and type IV collagen aligned in a gradient manner. We can assume that mesenchymal cells with different functions are also located in a graded way in accord with the graded distribution of collagen supramolecular aggregates. It was already known that proliferation, shape, migration, gene expression, and responses to growth factors of the cultured fibroblasts were greatly affected by the type I collagen fibrils. Other differentiated cells such as endothelial cells and smooth muscle cells are surrounded by type IV collagen and/or type V collagen more closely. The reconstituted type IV collagen aggregates in a gel form were tested as culture substrates for these differentiated cells. Of particular interest was the examination of myofibroblasts that are thought responsible for the pathogenesis of fibrotic or cirrhotic tissues. It was found that the type IV collagen gel appeared to restore the behaviors of smooth muscle-related cells including the myofibroblasts into the contractile stage. The whole cells cultured on the type IV collagen gel were totally quiescent and formed a multicellular network. The findings suggest that interrelationships in a graded way between differentiated cells and the specific collagen aggregates might be involved in the homeostasis of tissue structure and function.

    • Author Affiliations


      Toshihiko Hayashi1 Kazunori Mizuno2 Motohiro Hirose2 Koichi Nakazato2 Eijiro Adachi1 Yasutada Imamura2 Hiroaki Kosugi2 Kiwamu Yoshikawa2

      1. Department of Anatomy and Cell Biology, Kitasato University School of Medicine, Kitasato, Sagamihara, Kanagawa - 228, Japan
      2. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo - 153-8902, Japan
    • Dates

  • Journal of Chemical Sciences | News

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