• Selective quenching of tryptophanyl fluorescence in bovine serum albumin by the iodide ion

    • Fulltext


        Click here to view fulltext PDF

      Permanent link:

    • Keywords


      Tryptophanyl fluorescence; selective quenching; bovine serum albumin; iodide; modified Stern-Volmer equation

    • Abstract


      Modified Stern-Volmer equation is obeyed by bovine serum albumin (BSA)-iodide system showing selective quenching of tryptophanyl fluorescence of BSA. The fraction of accessible protein fluorescence is 0.56 and the effective Stern-Volmer constant is 290 M-1 at pH 7.4 in 0.005 M phosphate buffer at 25°C. Collisional quenching is operative both in the BSA -I−1 system and the model system, tryptophan-I−1. It is supported by the observed relationship between the ratio of quenching rate constants (kq) and diffusion coefficients and alsokq with bulk viscosity.

    • Author Affiliations


      Meenakshi Maruthamuthu1 G Selvakumar1

      1. Department of Physical Chemistry, University of Madras, Guindy Campus, Madras - 600025, India
    • Dates

  • Journal of Chemical Sciences | News

© 2021-2022 Indian Academy of Sciences, Bengaluru.