• Applications of synthetic oligoribonucleotide analogues in studies of RNA structure and function

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    • Keywords


      Oligoribonucleotides; modified nucleosides; ribozyme; protein-RNA recognition

    • Abstract


      The study of RNA structure and function has been considerably aided by the development of methods for the chemical synthesis of oligoribonucleotides into which have been incorporated modified nucleosides carrying site-specific alterations. Such modifications are designed to eliminate or alter individual functional groups in the RNA which potentially can take part in hydrogen-bonding or other non-covalent interactions. Comparison of the properties of the modified RNA with unmodified RNA models allows conclusions to be drawn concerning the importance or otherwise of specific functional groups within the RNA. The methods have been applied to studies of RNA structure, RNA catalysis, and interactions of RNA with proteins.

    • Author Affiliations


      Jane A Grasby1 Clare E Pritchard1 2 Michael J Gait1

      1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge - CB2 2QH, UK
      2. Department of Biochemistry, University of Oxford, Oxford - OX1 3QU
    • Dates

  • Journal of Chemical Sciences | News

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