The application of Fourier transform infrared spectroscopy and laser Raman spectroscopy for obtaining structural information about the subtle changes occurring at the molecular level following leprosy, pulmonary tuberculosis, infected burns, epilepsy and Duchenne muscular dystrophy (DMD) are reported in this paper. It is shown that IR spectra exhibit specific signals for each disease. Results of the observed bands of hemoproteins are compared with those of normals. Raman spectra of IgG reveal that alpha-helical conformations are introduced partially in case of the proliferation ofMycobacterium leprae. These results are supported by our IR studies. Typical signals of perturbed stretching region are seen in the pulmonary tuberculosis cases. Disappearance of a few normal bands and strong peaks in the hydrocarbon region characterizes epileptic disorder. Perturbations in the Raman spectra of erythrocyte ghosts are identified following gram-negativePseudomonas aeruginosa infection. Investigations of pathological changes following the onset of body disorders at the molecular level are reported using IR and Raman spectroscopic techniques.
Volume 131 | Issue 7
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