• Solid phase synthesis of the signal sequence ofE. coli alkaline phosphatase

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jcsc/097/01/0071-0075

    • Keywords

       

      Signal sequence; solid phase synthesis; transesterification; partition chromatography

    • Abstract

       

      A synthetic peptide corresponding to the signal sequence ofE. coli alkaline phosphatase has been synthesized by the solid phase method employing the transesterification method of cleavage from the resin. The protected peptide obtained after cleavage was purified to homogeneity by column chromatography on silica gel, followed by partition chromatography on SephadexLH-20 using organic solvents like chloroform and methanol as eluants.

    • Author Affiliations

       

      G Laxma Reddy1 R Nagaraj1

      1. Centre for Cellular & Molecular Biology, Uppal Road, Hyderabad - 500 007, India
    • Dates

       
  • Journal of Chemical Sciences | News

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