• Chemical approaches of penicillin allergy—III - Isolation of the penicillin-free carrier receptor protein (CRP) on a polymeric 7-deoxy penicillin analogue template and its role in penicillin immunogeneses in rabbit and man

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      https://www.ias.ac.in/article/fulltext/jcsc/087/01/0001-0012

    • Keywords

       

      Penicillin allergy; immunology; receptor; affinity chromatology; antigen-antibody interaction; hapten

    • Abstract

       

      Specific penicillin-carrier receptor proteins (CRP) have been isolated from the sera of penicillin allergic rabbits and human subjects in the unconjugated native state in electrophoretically homogeneous form by employing a synthetic polymeric affinity template containing the 7-deoxy analogue of penicillin G. The synthesis of the 7-deoxy analogue has been described. In this affinity system the antipenicillin-antibody is desorbed by 0·9M thiourea and the CRP in 8M urea. The CRP after incubation with penicillin is converted into the full-fledged antigen. Studies on the origin of CRP and the nature of antibody as well as comparative studies on the properties of the rabbit antibody and those of antibodies elicited by a BSA-BPO conjugate are reported.

    • Author Affiliations

       

      C V Nataraj1 Chitra Mandal1 P K Bhattacharyya1

      1. Department of Organic Chemistry, Indian Institute of Science, Bangalore - 560 012
    • Dates

       
  • Journal of Chemical Sciences | News

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