1. Home
    2. Journals
    3. Journal of Biosciences
    4. Volume 46
    5. All articles

    • MiR-296-3p inhibits cell proliferation by the SOX4-Wnt/β-catenin pathway in triple-negative breast cancer

      DUO TIAN LAIFU LUO TAO WANG JIAN QIAO

      Article Volume 46 Published: 12 November 2021 Article ID 0098

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/046/0098

    • Keywords

       

      TNBC; miR-296-3p; Wnt/beta-catenin; SOX4

    • Abstract

       

      MicroRNAs (miRNAs) have been demonstrated to play critical roles in the tumorigenesis of triple-negative breast cancer (TNBC). In this work, we addressed the specific role of miR-296-3p in TNBC. The levels ofmiR-296-3p and SOX4 were determined using RT-qPCR. The function of miR-296-3p overexpression onTNBC cell proliferation, migration, invasion, cancer stem cell (CSC)-like properties, and Wnt pathwayactivation was investigated by MTT, EdU, wound healing, Transwell, sphere formation assays and westernblot. Mechanistic investigations, including luciferase reporter, RNA pull-down, and RIP assays, were conductedto explore the regulatory mechanisms of miR-296-3p. We found that miR-296-3p was downregulatedin TNBC tissues and cells. Overexpression of miR-296-3p suppressed TNBC cell proliferation, migration,invasion, and CSC-like properties. Furthermore, miR-296-3p could bind to SOX4 and negatively modulateSOX4 expression. In addition, miR-296-3p was verified to inhibit Wnt/beta-catenin pathway by downregulatingSOX4. Moreover, overexpression of SOX4 or activation of Wnt pathway rescued the miR-296-3p upregulation-mediated suppressive effect on cellular processes in TNBC. In conclusion, miR-296-3p inhibits Wnt/beta-catenin pathway by targeting SOX4 and exerts anti-tumor effects in TNBC.

    • Author Affiliations

       

      DUO TIAN1 LAIFU LUO1 TAO WANG1 JIAN QIAO1

      1. Department of General Surgery, Hefei BOE Hospital, Hefei 230013, Anhui, China

    • Dates

       
      Published
      12 November 2021

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
      <

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2022-2023 Indian Academy of Sciences, Bengaluru.

Contact | Site index