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Rapid diagnosis of Leishmania infection with a portable loop-mediated isothermal amplification device
MADHU PURI HARSIMRAN KAUR BRAR NIMISHA MITTAL EVANKA MADAN RAJESH SRINIVASAN KAPIL RAWAT SRIJA MOULIK MITALI CHATTERJEE SAI SIVA GORTHI ROHINI MUTHUSWAMI RENTALA MADHUBALA
Article Volume 46 Published: 23 September 2020 Article ID 0092
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https://www.ias.ac.in/article/fulltext/jbsc/046/0092 -
Keywords
Diagnostic device ;LAMP ;Leishmania donovani ;post-kala azar dermal leishmaniasis ;visceral leishmaniasis -
Abstract
L. donovani is an intracellular protozoan parasite, that causes visceral leishmaniasis (VL), and consequently,post-kala azar dermal leishmaniasis (PKDL). Diagnosis and treatment of leishmaniasis is crucial for decreasingits transmission. Various diagnostic techniques like microscopy, enzyme-linked immunosorbent assays(ELISA) and PCR-based methods are used to detect leishmaniasis infection. More recently, loop-mediatedisothermal amplification (LAMP) assay has emerged as an ideal diagnostic measure for leishmaniasis, primarilydue to its accuracy, speed and simplicity. However, point-of-care diagnosis is still not been tested withthe LAMP assay. We have developed a portable LAMP device for the monitoring of Leishmania infection. TheLAMP assay performed using our device can detect and amplify as little as 100 femtograms of L. donovaniDNA. In a preliminary study, we have shown that the device can also amplify L. donovani DNA present in VLand PKDL patient samples with high sensitivity (100%), specificity (98%) and accuracy (99%), and can beused both for diagnostic and prognostic analysis. To our knowledge, this is the first report to describe thedevelopment and application of a portable LAMP device which has the potential to evolve as a point-of-carediagnostic and prognostic tool for Leishmania infections in future.
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Author Affiliations
MADHU PURI1 HARSIMRAN KAUR BRAR1 NIMISHA MITTAL1 EVANKA MADAN1 RAJESH SRINIVASAN2 KAPIL RAWAT2 SRIJA MOULIK3 MITALI CHATTERJEE3 SAI SIVA GORTHI2 ROHINI MUTHUSWAMI1 RENTALA MADHUBALA1
- School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
- Indian Institute of Science, Bengaluru 560 012, India
- Department of Pharmacology, Institute of Post-Graduate Medical Education and Research, Kolkata 700 020, India
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Dates
- Published
- 23 September 2020
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Supplementary Material
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Journal of Biosciences
Volume 48, 2023
All articles
Continuous Article Publishing mode
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Journal of Biosciences | News
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To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".
Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.
Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.
Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx
Posted on April 12, 2023< -
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Posted on July 25, 2019Click here for Editorial Note on CAP Mode
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